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Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) <t>Progesterone</t> remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).
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Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) <t>Progesterone</t> remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).
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Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) <t>Progesterone</t> remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).
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Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) <t>Progesterone</t> remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).
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Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) <t>Progesterone</t> remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).
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Abbott Laboratories 7k77 architect progesterone reagent kit
Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) <t>Progesterone</t> remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).
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Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) Progesterone remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).

Journal: iScience

Article Title: Steroid hormones dynamics during coral reproduction: Multi-year patterns in Acropora eurystoma from the Red Sea

doi: 10.1016/j.isci.2026.116205

Figure Lengend Snippet: Seasonal variation in steroid hormones and reproductive traits in Acropora eurystoma (2021–2023) (A) Estrogen peaked in March, declined through May and rose again toward spawning (GAMs: 26%–44% deviance explained, p < 0.001). (B) Progesterone remained stable during the reproductive season (January-July) but showed a marked October increase in 2022 (204% higher than reproductive season, p < 0.001). (C) Oocyte diameter followed a sigmoidal growth pattern to July (GAM: 87.6% deviance, p < 0.001); Developmental stages (I-V) adapted from Tan et al. (2020): stage I (35–75 μm), stage II (76–175 μm), stage III (176–285 μm), stage IV (286–385 μm), stage V (>386 μm). (D) Fecundity was stable across months (mean 18.6 ± 0.2 oocytes·polyp −1 ). Lines show monthly means by year (purple: 2021, orange: 2022, green: 2023, shaded areas: ±SE). Light blue shading in A and B indicates the typical spawning period (mid-June to mid-July).

Article Snippet: Total estrogen (100% specificity for E1, E2 and E3) was measured using an Eagle Biosciences kit (cat. ESG31-KO1), and progesterone was measured with an ALPCO direct saliva kit (cat. 11-PROHU-E01-SLV).

Techniques:

Relationships between steroid hormones and oocyte development in Acropora eurystoma (A) Estrogen correlated negatively with oocyte diameter (Spearman’s ρ = −0.32, p < 0.01, n = 86), indicating hormone decline during maturation. (B) Estrogen and progesterone correlated positively (ρ = 0.33, p < 0.001, n = 240), suggesting coordinated regulation. Colors denote sampling years (purple: 2021, orange: 2022, green: 2023). Dashed lines: linear regression; gray shading: 95% confidence intervals.

Journal: iScience

Article Title: Steroid hormones dynamics during coral reproduction: Multi-year patterns in Acropora eurystoma from the Red Sea

doi: 10.1016/j.isci.2026.116205

Figure Lengend Snippet: Relationships between steroid hormones and oocyte development in Acropora eurystoma (A) Estrogen correlated negatively with oocyte diameter (Spearman’s ρ = −0.32, p < 0.01, n = 86), indicating hormone decline during maturation. (B) Estrogen and progesterone correlated positively (ρ = 0.33, p < 0.001, n = 240), suggesting coordinated regulation. Colors denote sampling years (purple: 2021, orange: 2022, green: 2023). Dashed lines: linear regression; gray shading: 95% confidence intervals.

Article Snippet: Total estrogen (100% specificity for E1, E2 and E3) was measured using an Eagle Biosciences kit (cat. ESG31-KO1), and progesterone was measured with an ALPCO direct saliva kit (cat. 11-PROHU-E01-SLV).

Techniques: Sampling

Conceptual model summarizing annual reproductive and hormonal dynamics in Acropora eurystoma Estrogen (pink) peaks during early-mid gametogenesis (January-March) and declines with oocytes maturation. Progesterone (purple) remains low during gametogenesis but peaks in October, signaling cycle renewal. Oocyte development (green dashed) follows sigmoidal growth through spawning (Jun-Jul). The cycle comprises three phases: gametogenesis (Jan-Jun), spawning (Jun-Jul), and recovery/initiation (Jul-Dec).

Journal: iScience

Article Title: Steroid hormones dynamics during coral reproduction: Multi-year patterns in Acropora eurystoma from the Red Sea

doi: 10.1016/j.isci.2026.116205

Figure Lengend Snippet: Conceptual model summarizing annual reproductive and hormonal dynamics in Acropora eurystoma Estrogen (pink) peaks during early-mid gametogenesis (January-March) and declines with oocytes maturation. Progesterone (purple) remains low during gametogenesis but peaks in October, signaling cycle renewal. Oocyte development (green dashed) follows sigmoidal growth through spawning (Jun-Jul). The cycle comprises three phases: gametogenesis (Jan-Jun), spawning (Jun-Jul), and recovery/initiation (Jul-Dec).

Article Snippet: Total estrogen (100% specificity for E1, E2 and E3) was measured using an Eagle Biosciences kit (cat. ESG31-KO1), and progesterone was measured with an ALPCO direct saliva kit (cat. 11-PROHU-E01-SLV).

Techniques: